http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103543219-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f22b137b7f2f9f0a2fc6a538b40f2cd0 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_416e667ddc43b7c1ff22c061d2f167c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0948d0885a04cd14018ffec9eddc6e54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_473d318c535c75dfa5fc20a4e6a835b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_912e4e416bf0635fcce05391666b812b |
| publicationDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103543219-B |
| titleOfInvention | Method for extracting cyanobacteria toxin from urban eutrophic cyanobacterial bloom water |
| abstract | The invention discloses a method for extracting cyanobacteria toxin from urban eutrophic cyanobacterial bloom water. The method comprises the following steps: (1) adding methyl alcohol and glacial acetic acid into to-be-extracted water to ensure that the final volume content of the methyl alcohol is 40% and the final volume content of the glacial acetic acid is 3-5%, and carrying out continuous ultrasonic treatment at the frequency of 300 hertz for 15-20 minutes; (2) regulating the pH (potential of Hydrogen) of the water sample subjected to the ultrasonic treatment of the step (1) to 3, filtering by using a filter membrane with the pore size of 0.45 micrometers, centrifuging the filtrate in the weight of 6500g for 5-10 minutes and taking a supernatant; (3) carrying out solid phase extraction on the supernatant obtained in the step (2) by using a solid phase extraction column, leaching by using a methyl alcohol aqueous solution with the volume content of 40%, and eluting by using a methyl alcohol aqueous solution with the volume content of 95% to obtain eluent containing the cyanobacteria toxin; (4) obtaining the cyanobacteria toxin from the eluent obtained in the step (3). According to the method disclosed by the invention, by adopting a solid phase extraction technique, two main isomers of the cyanobacteria toxin in urban lake water, namely MC-LR and MC-RR, are continuously enriched, purified and extracted; the method has the characteristics of high product purity, easiness for operation, low cost and the like. |
| priorityDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.