http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103525864-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103525864-B |
| titleOfInvention | The method of soybean transformation |
| abstract | The present invention relates to a kind of method of soybean transformation, comprising: after Germination of Soybean Seed, remove cotyledon and rough leaf, obtain exposed meristematic tissue; Described exposed meristematic tissue is inoculated on the pre-treatment substratum containing phytokinin and carries out pre-treatment; Agrobacterium infects described pretreated meristematic tissue block.The method of soybean transformation of the present invention makes public for the first time pretreated soybean meristematic tissue block as explant material; By carrying out the phytokinin process of high density to explant material before Agrobacterium is infected, the meristematic tissue of soybean is made to be in active state, be easy to the insertion of foreign gene, become large by merismatic increment simultaneously, make infection processs convenient operation more; In Agrobacterium infection processs, add ultrasonication, the efficiency making foreign gene insert Plant Genome increases greatly, and the transformation efficiency of soybean can reach 10%; Transformation period is short simultaneously, mosaic is few, it is low to transform cost. |
| priorityDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 186.