http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103525756-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26ed25f3feac102eae4ed421389c340f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2013-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8cedf4409dcf3c632fbb26b0bb47053b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e9959ed2159a607210732bb9ad57d79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8893d3f4a75c34f823b2e703d723bca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5c678267a5045367ce8e9f7ebb7a7e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60b15b2b1a955e99a09d1626bcc5f9a5 |
| publicationDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103525756-B |
| titleOfInvention | Method for separating and culturing primary chicken hepatocytes |
| abstract | The invention discloses a method for separating and culturing primary chicken hepatocytes. According to the method, EGTA (Ethylene Glycol Tetraacetic Acid) with low hepatotoxicity is added into a perfusate A to loosen the junction between hepatocytes, so that the dispersion degree of the to-be-separated hepatocytes is increased; double resistance is added into the perfusate A, a perfusate B and D-Hanks flushing fluid, so that the pollution is further prevented; and circulating perfusion digestion is adopted during digestion perfusion, so that the perfusion cost is greatly reduced. A serum-free cultural method of the chicken hepatocytes is established; compared with a traditional serum cultural method, the method disclosed by the invention has the same effect on the aspects of cell quantity, activity, function and the like, so that the problems caused by the existence of serum are solved and a foundation is laid for the substance metabolism study and biological product preparation on the basis of the chicken hepatocyte culture. |
| priorityDate | 2013-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.