http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103509761-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_374e1088d4ea8f45a9b5396d4302ec03 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_729986fb0f531eb6b4bd0f1b51be0c8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a00471a3f5aa0149f5663c33880f9556 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52741676c47fedd867d4308726879127 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aca8adeda33d819b11983ff4d60d0aeb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4901885944051605833f4efc81bd35f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8841436c0ef8458a63d017678489d1b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6bedde92743bfdafe69f9780ca8dd63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81c354d8de3dd133933fb197a8bafd4a |
| publicationDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103509761-B |
| titleOfInvention | Recombinant porcine pseudorabies virus strain used for expression of porcine circovirus type II (PCV2) ORF2 gene, and preparation method thereof |
| abstract | The invention discloses a recombinant porcine pseudorabies virus (Herpesviridae) strain used for expression of porcine circovirus type II ORF2 gene. Preservation number of the recombinant porcine pseudorabies virus strain is CCTCC No.V201315. According to the preparation method, PCV2ORF2 gene is inserted into common carrier PG so as to construct transferring plasmid PGO; monolayer ST cells are inoculated with PRVTK<->/gG<->/gE<-> virus by 2h of adsorption; ST cells are transfected with plasmid PGO, wherein fusion degree of the monolayer ST cells is 80 to 90%; the recombinant porcine pseudorabies virus PGO strain is obtained by plaque purification, and is used for immunization of mouse. It is shown by results that commercial PCV2 inactivated vaccine and a group immunized by the recombinant porcine pseudorabies virus PGO strain are both capable of inducing specific humoral immune response of mouse, antibody titers of both the commercial PCV2 inactivated vaccine and the group are obviously higher than that of a group immunized by DMEM medium, and difference is significant (p<0.05). It is shown by the results of mouse lymphocyte proliferation test that specific ceullar immune response caused by the group immunized by the recombinant porcine pseudorabies virus PGO strain is more obvious than that caused by the commercial PCV2 inactivated vaccine and the group immunized by DMEM medium, and difference is obvious (p<0.05). In addition, the group immunized by the recombinant porcine pseudorabies virus PGO strain is capable of resisting severe attack by PCV2. Therefore application of the recombinant porcine pseudorabies virus strain in development of a novel PCV2 vaccine is possible. |
| priorityDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 133.