abstract |
The invention relates to a female cynoglossus semilaevis specific protein CSW2, whose amino acid sequence is represented by the SEQ ID No.2; the sequence of the nucleotide, which encodes the female cynoglossus semilaevis specific protein CSW2, is represented by the SEQ ID No.1. The external recombinant protein of the CSW2 gene can prominently improve the aromatizing enzyme expression level of female cynoglossus semilaevis fry related genes, namely fox12 and P450, has a biological activity which can promote female-related gene expression, and at the same time has a biological activity that can reduce the expression level of male-related gene SOX9. The recombinant expression product of CSW2 can be used as a feed addictive, which has the function of promoting the growth of female gonad and has an effect of improving the female fish ratio. So the CSW2 gene provided by the invention has a potential application prospect on aspects of cynoglossus semilaevis gender control and improvement on the female fry ratio. |