http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103497936-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B09C1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B09C1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
filingDate | 2013-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103497936-B |
titleOfInvention | Remove zymin and the application of Ortho Nitro Benzaldehyde |
abstract | The invention belongs to enzyme genetically engineered and enzyme engineering field, relate to a kind of zymin and application of removing Ortho Nitro Benzaldehyde.To remove a zymin for Ortho Nitro Benzaldehyde, it is characterized in that: described zymin is the aqueous solution or buffered soln, comprise a kind of O-nitrobenzaldehyde degrading enzyme, is its aminoacid sequence as SEQ? ID? shown in No.1, and final concentration is the CaCl of 0.1-0.3mMn 2 , pH value of reaction system is 6.5-8.0.When zymin is buffered soln, this buffered soln is generally 50-100mM sodium phosphate buffer or the potassium phosphate buffer of pH value 7.2-7.7.The pure enzyme content of described O-nitrobenzaldehyde degrading enzyme is 0.03-0.1mg/mL.Zymin of the present invention, by preferably adding metallic compound, makes zymin enzyme of the present invention live high, degraded ONBA excellent effect; The microbiological deterioration enzyme adopted adopts genetic engineering means preparation, and cost is low, is conducive to large-scale promotion; Adopt enzymic degradation ONBA, not only effective, and simple to operate, safe, required reaction conditions is gentle, does not form secondary pollution. |
priorityDate | 2013-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.