http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103497248-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103497248-B |
| titleOfInvention | A kind of method of isolated and purified antibody from cells and supernatant |
| abstract | The invention discloses a kind of method of isolated and purified antibody from cells and supernatant.The first method of isolated and purified antibody from cells and supernatant that the present invention provides, in turn includes the following steps: (1) takes can be with the cells and supernatant of the cell of secretory antibody；(2) affinity chromatograph is carried out；(3) cation-exchange chromatography is carried out；(4) anion-exchange chromatography is carried out；It is characterized in that: after each chromatographic step, do not carry out the displacement of buffer, directly carry out next chromatographic step.The method that the present invention provides has step simplification, DNA purity height, and impurity content is low, antibody yield advantages of higher, has substantial worth for realizing the Produce on a large scale of antibody purification. |
| priorityDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.