| abstract |
The invention relates to an enzyme-linked immunosorbent assay (ELISA) detection method, which can be applied to enzyme-linked immunosorbent assay (ELISA) detection of perfluorooctane sulfonated (PFOs) residual in the environment. The method comprises following steps: step one, synthesizing and identifying PFOs artificial antigen; step two, preparing and purifying PFOs antibody; step three, establishing PFOs indirectly competing ELISA quantitative detection method; step four, substituting PFOs with 9 PFCs with similar structures as that of PFOs, and then respectively measuring the cross-reactivity of the 9 PFCs with an antibody. The technology provides a method of rapidly detecting PFOs residual in the environment, better improves the detection sensitivity, guarantees the precision of the detection results, satisfy the requirements on PFOs residual detection at present, and is convenient for carrying out PFOs residual detection in the environment. |