| abstract |
The invention relates to an immunoaffinity chromatography-ultrahigh performance chromatography-mass spectrum combined rapid detection method, and especially relates to a novel technology of a detection method for perfluorinated compounds in dairy products or water bodies. The method comprises following steps: step one, synthesis of artificial antigens; step two, screening and identification of polyclonal antibodies; step three, development of an immunoaffinity column; step four, establishment and optimizing of serially-combined detection method of ultrahigh performance liquid chromatography and mass spectrum. The technology provides a rapid detection method for perfluorinated compound residuals in the environment, improves the detection sensitivity, and guarantees the detection accuracy. The method meets the requirements on detection of perfluorinated compound residuals, and is beneficial for carrying out detections of perfluorinated compounds in real samples in the environment. |