http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103468811-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_978a305ba442ec0a8b73bcaec1c55946 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75f1b0417d48dc4b8869c7f242d97735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c0136ecd0febfb3fceb7043c59ebd73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e08af748cf0386a195be4a7b962a0f2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0843b81f5b933db0921ab62ad8d9859c |
| publicationDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103468811-B |
| titleOfInvention | Yersinia enterocolitica virulence gene multiplex-PCR (Polymerase Chain Reaction) detection primer group and kit |
| abstract | The invention discloses a yersinia enterocolitica virulence gene multiplex-PCR (Polymerase Chain Reaction) detection primer group and a kit. The yersinia enterocolitica virulence gene multiplex-PCR detection primer group comprises primer pairs for detecting yersinia enterocolitica 16S rRNA (ribosomal Ribose Nucleic Acid) and detecting an adhesion invasion locus gene, a heat-liable enterotoxin A gene, an adhesin gene, a heat-liable enterotoxin B gene, a virulence activation factor gene and an O:3 serotype antigen coding gene relevant to the pathogenic yersinia enterocolitica. According to the invention, a multiplex-PCR detection method based on a common PCR platform is established, DNA extracted by separating an enriched liquid medium to be detected or flat-plate bacterial colony is subjected to multiple PCR reaction by utilizing the primer pairs obtained through analysis design, a PCR product is subjected to electrophoretic analysis after the reaction is ended so as to determine that whether the yersinia enterocolitica exists in a sample or not and determine a virulence gene relevant to the pathogenic yersinia enterocolitica in the sample and judge that whether the virulence gene is O:3 serotype or not. |
| priorityDate | 2013-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 217.