http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103468678-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-04 |
filingDate | 2013-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103468678-B |
titleOfInvention | High gas oil ratio peanut molecule marker and assisted Selection back cross breeding methods and applications thereof |
abstract | The present invention relates to a kind of high oleic acid molecular marker and utilize this mark to carry out back cross breeding method and the application thereof of assisted Selection.The variation specific primer sequences of molecule marker is: forward primer MITE-INS-F and reverse primer MITE-INS-R; It without variation specific primer sequences is: forward primer WILD-F and reverse primer WILD-R.Utilizing molecule marker to carry out back cross breeding comprises the peanut parent of frequency of genotypes AA BB, with genotype aabb parents, then backcrosses; Utilize the pair of primers amplification FAD2A gene fragment in genome, select containing the genotypic individual plant of Aa; Utilize the primer in peanut <i>ahFAD2B</iGre atT.GreaT.GT site to carry out PCR detection as mark, select containing the genotypic individual plant of Bb; Namely backcross all dominant for two marks containing the genotypic individual plant of AaBb and parent AABB, until selecting genotype from offspring is aabb, the high gas oil ratio individual plant that other economical character is consistent with recurrent parent, then selfing and expanding propagation.Molecule marker of the present invention, method is simple, and cost is low, and result is stablized; This molecule marker carries out back cross breeding, can significantly improve the accuracy of character determination, reduces breeding cost, improves breeding efficiency. |
priorityDate | 2013-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.