http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103451173-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fefb70c664408a9f0ee32b1eef68c901 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_853a68da6057db3e5a180a3cfc43ea85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bb431c6dca512ad375b2ea6caae31bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_defbbc6cf11004625cb8690f5b259eaf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_046a5e44060679a64ffd11933af62891 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1fab8f5d0558db46ee77fd95b344287 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0de3867821c78c9c4a67cd77150b84c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8294bc37bcb1b7d4c0f2446c48089330 |
| publicationDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103451173-B |
| titleOfInvention | Method for improving activity of pseudomonas aeruginosa elastase (PAE) by carrying out single-point mutation to delete salt bond in structure |
| abstract | The invention provides a method for improving activity of pseudomonas aeruginosa elastase (PAE) by carrying out single-point mutation to delete salt bond in structure. By adopting a site-directed mutagenesis technology, an Asp201 in Asp201-Arg205 for forming the salt bond 3 is mutated into Ala by deleting a single salt bond near a catalytic cavity; a mutant D201A is built, so as to improve the activity of the PAE. The method has significance on revelation of the structure and the functional relationship of M4 family metal proteases; meanwhile, a plurality of M4 family metal proteases are important medical and industrial enzymes, therefore, the method has an important guiding significance on molecular modification research and development of related new products of the family proteases. |
| priorityDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 366.