http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103451154-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
filingDate | 2012-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103451154-B |
titleOfInvention | There is cell strain and the establishment method thereof of anti-apoptotic and regulation of cell proliferation characteristic |
abstract | E1B-19K gene and BS-BV gene are inserted into EF-1 α promotor and the CMV promoter downstream of pBudCE4.1 carrier by the present invention respectively, build simultaneously, the carrier pBud-E1B-BS-BV of independent constitutive expression E1B-19K and BS-BirA-VP4.P27Kip1 fragment is connected in the response carrier of vitamin H inducible expression BS-Biotin-On, builds the p27Kip1 inducible expression carrier pc-Hy-4BSOB-minp27 in response to vitamin H.By above-mentioned two carrier cotransfection HEK293 cells, carry out mono-clonal by limiting dilution assay, obtain the HEK293 cell strain 18-HEP with Ameliorative character. |
priorityDate | 2012-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.