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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
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filingDate 2012-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103451154-B
titleOfInvention There is cell strain and the establishment method thereof of anti-apoptotic and regulation of cell proliferation characteristic
abstract E1B-19K gene and BS-BV gene are inserted into EF-1 α promotor and the CMV promoter downstream of pBudCE4.1 carrier by the present invention respectively, build simultaneously, the carrier pBud-E1B-BS-BV of independent constitutive expression E1B-19K and BS-BirA-VP4.P27Kip1 fragment is connected in the response carrier of vitamin H inducible expression BS-Biotin-On, builds the p27Kip1 inducible expression carrier pc-Hy-4BSOB-minp27 in response to vitamin H.By above-mentioned two carrier cotransfection HEK293 cells, carry out mono-clonal by limiting dilution assay, obtain the HEK293 cell strain 18-HEP with Ameliorative character.
priorityDate 2012-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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