http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103451148-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103451148-B |
| titleOfInvention | People's normal bronchial epithelial cell and primary separation and Culture thereof and Secondary Culture method and purposes |
| abstract | The invention discloses people's normal bronchial epithelial cell and primary separation and Culture thereof and Secondary Culture method and purposes.The name of this cell is people's normal bronchial epithelial cell HNBEC/HL-001, and deposit number is CCTCC NO:C201311.The method of its primary separation and Culture is: remove fat in cancer beside organism's sample of Operation for Lung Cancer excision, after digestion, add Dispase and DNase I effect, by filtration, centrifugal collecting cell, then use HL substratum re-suspended cell, inoculation culture.The method of its Secondary Culture is: during cell proliferation to 70 ~ 90% abundance, with pancreas enzyme-EDTA digestion, then neutralizes with DMEM; Centrifugal collecting cell, with HL substratum re-suspended cell, inoculation culture.Cell of the present invention can be used for the Physiologic Studies of human normal cell line, and external Normocellular drug toxicity research and detection, segmental bronchus and lung relative disease comprise the study of pathogenesis of lung bronchogenic carcinoma. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106497866-A |
| priorityDate | 2013-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 144.