http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103439508-B

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577
filingDate 2013-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8940e00ee1824d57d3a4dbc5651e0e5e
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publicationDate 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103439508-B
titleOfInvention Indirect competitive enzyme linked immunosorbent assay kit for detecting chromium ions as well as preparation and detection methods thereof
abstract The invention relates to an indirect competitive enzyme linked immunosorbent assay (elisa) kit for detecting chromium ions as well as preparation and detection methods thereof. A trivalent chromic ion envelope antigen-enveloped elisa plate, a trivalent chromic ion-resistant monoclonal antibody, a second enzyme-labeled antibody, a substrate color-developing solution, a stop solution, a trivalent chromic ion standard solution, a lotion concentrated solution and a sample processing solution are arranged in the kit. Samples are processed before detection so that Cr3<+> in the samples forms Cr<3+>-EDTA (ethylene diamine tetraacetic acid), and then the chromium ions are detected by the kit. The chromium ion enzyme linked immunosorbent assay kit can be used for detecting the trace amount of chromium ions, has the Cr<3+> detection sensitivity of 2ng/ml, has the characteristics of quickness, simplicity and convenience, sensitivity, specificity, economy and the like, has few detection steps, saves the detection time, and reduces the operation error; the detection cost is less than 1/20 of that of a physical and chemical analysis method, the timeliness is high, the field detection can be performed, and the kit is mainly used for screening large-batch samples of chromium ion pollution residues in environments, soil, water and foods.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018154383-A1
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