http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103439318-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fefd66fdbc3870bbaa8e5a4f87bdb0a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate | 2013-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7edbcaec080c75b6dc9417b0096865b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1208022a8a7a4c01aac1dfa4080a5277 |
| publicationDate | 2015-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103439318-B |
| titleOfInvention | Detection method for lead ion in environment |
| abstract | The invention discloses a detection method for a lead ion. The method comprises the following steps: respectively diluting a sequence 1 and a sequence 2 in a sequence table with a buffer solution and then carrying out mixing and hybridization to obtain a nuclease solution, wherein a mol ratio of the sequence 1 to the sequence 2 is 1: 1 to 3: 1; adding a pretreated sample to be detected into the nuclease solution, carrying out standing to allow nuclease to completely react with lead ions and then adding a sequence 3, wherein a mol ratio of the the sequence 3 to the sequence 2 is 1: 1 to 3: 1; carrying out standing again and then adding hemin chloride so as to obtain a solution A; slowly mixing the solution A with a solution B according to a volume ratio of 1: 2 to 2: 1 and detecting chemiluminescent intensity of a mixed solution during a mixed reaction, wherein the solution B is an aqueous buffer solution containing luminol, hydrogen peroxide and p-lodophenol; and calculating the concentration of the lead ions in the sample to be detected based on the chemiluminescent intensity of the mixed solution. The detection method provided by the invention is used for detection of the content of lead ions, has the advantages of high sensitivity, good selectivity, superior reproducibility and simple and easily practicable operation and can be directly applied to detection of lead ions in an environment. |
| priorityDate | 2013-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 187.