http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103436548-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
filingDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103436548-B |
titleOfInvention | Utilize the method for Tet-off inducible expression high flux screening HIV-1 integrase inhibitor |
abstract | The invention discloses a kind of method utilizing Tet-off inducible expression high flux screening HIV-1 integrase inhibitor, comprise and HIV-1 integrase gene and reinforced green fluorescence protein gene fusion cloning are entered carrier for expression of eukaryon pTRE-2hyg, by the plasmid extraction kit of transfection rank, extracting pTRE-2hyg-EGFP-IN plasmid, for cell transfection assays, by recombinant plasmid pTRE-2hyg-EGFP-IN transfection HeLa? Tet-Off? Advanced? Cells, the expression of induced fusion albumen, carry out drug screening, the medicine of intergrase apoptotic nueleolus can be suppressed to be judged to be positive findings.The present invention utilizes Tet-off inducible expression, and for intergrase-LEDGF/p75 target spot, high flux screening integrase inhibitor, reliable and stable the screening HIV-1 integrase inhibitor of energy, the screening for integrase inhibitor greatly improves.Material source of the present invention is extensive, and Be very effective, reproducible, application prospect is good. |
priorityDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.