| abstract |
The invention provides a method of improving PAE specific activity by deleting a single salt bond. According to the invention, by utilizing a site-directed mutagenesis technology, and through the deletion of the single salt bond next to a catalyzing chamber, the activity of PAE is improved; the method not only has significant for disclosing the structure and function relation of an M4 family metalloprotease, and meanwhile, as a plurality of M4 family proteases are significant enzymes for medical and industrial use; therefore, the method also has a significant guiding significance for a molecular modification search of the family protease and the development of a related new product. |