http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103432579-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5ed85c5a886b8df4923d9a2596262902 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-08 |
| filingDate | 2013-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_547a10d9b9f32085be2b30d87ca5243d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ea794f8779e86c0c7c80f070f7baca1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0a98a8f17b15387f9c2ebf207e30d45 |
| publicationDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103432579-B |
| titleOfInvention | Preparation method of lamb-dysentery clostridium perfringens type B toxoid vaccine |
| abstract | The invention relates to a preparation method of a preparation method of a lamb-dysentery clostridium perfringens type B toxoid vaccine. The preparation method comprises the following steps of inoculating the clostridium perfringens type B in a thioglycollate fluid culture medium for enriched culture under the condition of an anaerobic environment at 38 DEG C for 14 h; adding the culture to a culture medium of tryptone, dextrin, yeast extract and L-arginine which are dissolved by a buffer solution, carrying out shaking culture in the anaerobic environment for 5 h, and then performing 3800 g centrifuging at 4 DEG C for 15 min, and filtering to remove bacteria in a Seitz filter; adding a toxoid inactivated previously to a sterile white oil adjuvant in the ratio of 1: 1, and adding 0.01% of merthiolate, thereby obtaining the toxoid vaccine. The vaccine is free of toxic and side effects, high in valence, strong in immunizing power, and capable of effectively preventing and controlling clostridium perfringens type B induced lamb dysentery and enterotoksemia or necroticenteritis of sheep, goat, foal and calf. |
| priorityDate | 2013-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.