http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103421831-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2013-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103421831-B |
| titleOfInvention | For improving mosaic gene and the construction process thereof of the hepatocellular physiological CYP3A4 expression of vitro culture of human |
| abstract | The present invention discloses a kind of for improving the mosaic gene and construction process thereof that the hepatocellular physiological CYP3A4 of vitro culture of human expresses; Described mosaic gene, comprises the activation domain sequence of people's nf Pregnane X Receptor full length gene sequence and wild type P53 gene.Also has a kind of recombinant eukaryon expression vector, it mosaic gene described before comprising; A kind of construction process of foregoing recombinant eukaryon expression vector, wherein, it comprises the following steps: the activation domain sequence of cloning described people's nf Pregnane X Receptor full length gene sequence and wild type P53 gene from the cDNA in normal people's primary hepatocyte source respectively; Two sections of sequence directed clonings abovementioned steps obtained, between the β-globin/IgG chimeric intron sequence and SV40Late poly (A) signal sequence of described PCI-neo mammalian expression vector, obtain recombinant eukaryon expression vector.The present invention can significantly improve the hepatocellular physiological CYP3A4 of vitro culture of human and express. |
| priorityDate | 2013-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 712.