http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103412128-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 |
| filingDate | 2013-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9b6b64726024a47a6272f3b15efc56e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6996925bdd6217e7e26db9c9c3d022f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_188d2da183f02074821a2551cff8cee7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30db370cbf6a08a5967b9a881a3da63f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e7ff456bfcbbb44d9defb106d934ad0 |
| publicationDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103412128-B |
| titleOfInvention | Method for detecting toxin T-2 in viscera of animals by using immune chip |
| abstract | The invention discloses a method for detecting toxin T-2 in viscera of animals by using an immune chip. The method comprises the steps of (1) preparing a complete antigen point of toxin T-2 on a chip; (2) carrying out immune-chip technical research by adopting a competition method, namely adding a mixture, prepared from a single antibody of toxin T-2 and a standard (or a tagged recovered sample solution) which are of an equal volume, to the chip during detection; and (3) adding a Cy5-tagged secondary antibody so as to form an antigen-single antibody-secondary antibody compound for a substance to be detected, wherein fluorescent signals are weakened along with the increase of the concentration of small molecules, so that the aim of detection is achieved. The method is applied to the detection on residual toxin T-2 in the pork liver, the recovery percent and average tagged recovery percent for toxin T-2 in the pork liver are 69.91-87.80%, the coefficient of variation is 4.88-12.56%, and a chip immunoassay method is simple and fast and is high in sensitivity, high in throughput and small in sample loaded quantity, so that the method is applicable to residue analysis on toxin T-2 in the viscera of the animals. |
| priorityDate | 2013-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.