http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103409563-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0328dc1833863fe13dfa746048bec03 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af12f7919e1672e69f66077620418f2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_797f204e3a04558b0edccce61ec64993 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5934bb9d291b1140eb9b33f0b81925a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16491574bdb0186713d9e2e8c0b94f81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b533b8b03e43318f53357e9890463fa0 |
| publicationDate | 2014-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103409563-B |
| titleOfInvention | RT-LAMP (reverse transcription-loop-mediated isothermal amplification) detection kit for infectious pancreatic necrosis virus of rainbow trout |
| abstract | The invention relates to a kit for rapidly detecting an infectious pancreatic necrosis virus of rainbow trout by utilizing a loop-mediated isothermal amplification (LAMP) technique and a detection method of the infectious pancreatic necrosis virus of rainbow trout. The kit comprises reaction liquid A and reaction liquid B, wherein the reaction liquid A contains 10*isothermal reaction buffer liquid, 5U/microliter of AMV, 20U/microliter of Rnasin, 8U/microliter of Bst DNA (Deoxyribose Nucleic Acid) polymerase, 10mM of dNTP (deoxy-ribonucleoside triphosphate), 25mM of magnesium sulfate, 20 micrometers of inner primers 1, 20 micrometers of inner primers 2, 10 micrometers of outer primers 1, 10 micrometers of outer primers 2, 30 micrometers of circular primers 2 and 5M of lycine; the reaction liquid B is 1000*fluorochrome SYBR (Synergy Brands) GreenI. According to the kit, the rapid detection of the infectious pancreatic necrosis virus is realized by carrying out methods of the RNA (Ribose Nucleic Acid) extraction in tissue samples or cell culture liquid, the loop-mediated isothermal amplification of the infectious pancreatic necrosis virus, the color development and detection of amplified products and the like. With the adoption of the technique, the defects that the detection time is long, the operation is complicated, expensive instruments are required, and the like in the existing technical detection are overcome, and the technique is suitable for rapid spot detection in livestock farms, veterinary stations and the like. |
| priorityDate | 2013-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.