http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103409410-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e9297c8f247b3724a04ec3c3130f182 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2013-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba77dca90a858cab44171b398477af29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62ecb62dd33add887f2f5d1802bf8804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0004addd72ec84845cb9ea942e0855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77cf678d02d72c867f8a1c08ad72f410 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3e2cd25fdd28eaa3e8f9c66e7103086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db7b1bb1531113d071e656c38a0d6b29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c8ffb114feb84b0f66263b1da3010f6 |
| publicationDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103409410-B |
| titleOfInvention | Method for extracting the total microbial genome in vinegar fermentation process |
| abstract | The invention relates to a general method for extracting the total microbial genome form traditional vinegar fermentation process samples, and belongs to the technical filed of molecular biology. A technical issue to be solved by the invention is to provide a method for extracting the total microbial genome from traditional vinegar samples in different states comprising solid state fermentation, semi-solid state fermentation and liquid state fermentation. The method comprises steps of pretreating the vinegar fermentation samples, extracting microbial genome DNA, removing impurities, rinsing and concentrating. The method for extracting relates to in-situ extraction. The sample amount needed is less than 5 g. Addition of preparations such as protease, cellulase, helicase, etc. is not needed. The genome extracted has high purity and high quality, and can be directly used for subsequent molecular fingerprint analysis, metagenomics analysis, PCR (polymerase chain reaction) amplification of targeted genes and clone library construction. |
| priorityDate | 2013-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.