http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103396984-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
filingDate | 2013-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103396984-B |
titleOfInvention | A kind of method utilizing the progenitor cell of skin-derived to prepare tissue engineering spinal cord |
abstract | The invention discloses a kind of method utilizing the progenitor cell of skin-derived to prepare tissue engineering spinal cord, comprise the steps: 1), be separated SKPs, go down to posterity to obtain SKPs primary cell; 2) increase in the SKPs primary cell immigration amplification culture medium, separation obtained; 3), prepare tissue engineering spinal cord, by tissue engineering spinal cord material saturated aqueous solution, be added drop-wise in the normal saline solution containing Neurotrophin-3 (NT3), vitamin A acid and Neuregulin; By the SKPs after amplification, infect with brain-derived neurotrophy hormone adenovirus expression vector, seed cells on engineering spinal cord material, cultivate.In method of the present invention, tissue engineering spinal cord can promote cell proliferation effectively, and the ratio of its neuralward unit and oligodendrocyte differentiation is 4.8% and about 1.5%, far away higher than 1.8% and 0.5% of simple timbering material. |
priorityDate | 2013-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 207.