http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103396977-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P7-04 |
| filingDate | 2013-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103396977-B |
| titleOfInvention | Bacillus thuringiensis engineering bacterium for killing coleopteran pests as well as preparation method and application thereof |
| abstract | The invention discloses a bacillus thuringiensis engineering bacterium for killing coleopteran pests as well as a preparation method and application thereof. The preservation number is CCTCCM2013259. The preparation method comprises the following steps of: A, cloning insecticidal crystal protein gene cry3Aa8 from a Bt bacterial strain YC-03 for killing the coleopteran pests, inserting the gene into a disaggregation carrier pHT304/res and obtaining a recombined plasmid pHT3Aa8/res, converting the DNA (deoxyribonucleic acid) of the pHT3Aa8/res into a Bt aphakic mutant strain BMB171 through electric shock, screening so as to obtain transformants, shifting an auxiliary plasmid pBMB1200 into the transformant, selecting a positive transformant not growing on an Amp/Erm flat panel but growing on an LB flat panel, breeding in an LB fluid nutrient medium, and selecting a single colony growing on the LB flat panel but not growing on a tetracycline flat panel; B, taking plagiodera versicolora and the larva of phaedon brassicae baly as the test pest, carrying out toxicity bioassay, comparing the toxicity lethal dose LC50, and screening the high-toxicity engineering bacterium strain Ace-38. The method is practicable, and is simple and convenient to operate, the bacillus thuringiensis engineering bacterium has high effect on killing a plurality of coleopteran pests such as plagiodera versicolora, potato beetles, vegetable phaedon brassicae baly, aulacophora indica and the like, and the bacterium has good fermenting property, and has development and application prospects. |
| priorityDate | 2013-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.