http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103389382-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90348a429ffa25bbd39a647ab0d9173e |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb74b4a6be820b95c546c1a602f5ca34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6271f66c794adee101cb690a96dfd647 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27bf66dba5f285c758816db26b311508 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c39fa18dde14e23c94152f1b24761b69 |
publicationDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103389382-B |
titleOfInvention | Signal-enhanced test strip biosensor for detecting histone methylation |
abstract | The invention relates to a signal-enhanced test strip biosensor for detecting histone methylation. The test strip biosensor comprises a bottom plate with viscidity, a nitrocellulose membrane, a colloidal gold pad, an absorbent paper, and a sample pad, wherein the nitrocellulose membrane is attached to the middle part of the bottom plate; a monoclonal antibody of anti-histone H3 and a goat-anti-rat IgG antibody are sprayed on the nitrocellulose membrane; a detection line and a quality control line are respectively formed; the colloidal gold pad is attached to the bottom plate at one side of the nitrocellulose membrane; gold nanoparticle solution doubly marked by c-DNA (deoxyribonucleic acid) and the monoclonal antibody of anti-histone H3 is sprayed on the colloidal gold pad; the absorbent paper is attached to the bottom plate at the other side of the nitrocellulose membrane; the sample pad is attached to the bottom plate; histone extract is firstly dropwise added to the sample pad in detection; then an enhanced probe formed by DNA and gold nanoparticles in a coupling manner is dropwise added. By adopting the test strip biosensor provided by the invention, the histone methylation condition can be simply and rapidly detected; a detection signal is further amplified by the enhanced probe. |
priorityDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.