http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103389380-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2013-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103389380-B |
| titleOfInvention | The double-antibody sandwich elisa detection method of a kind of goose FSH |
| abstract | The double-antibody sandwich elisa detection method of a kind of goose FSH, comprise the kit step that double-antibody sandwich elisa detection method relates to, first the FSH α of goose and the mature polypeptide coding sequence of FSH beta subunit gene is cloned, build the prokaryotic expression carrier of the mature polypeptide coding sequence of FSH α and FSH beta subunit gene, express and purifying His-FSH α and GST-FSH beta fusion proteins, then using them for antigen acquisition goat-anti goose FSH α polyclonal antibody and rabbit anti-goose FSH β polyclonal antibody are as detection antibody and capture antibody; FSH α is connected with CTP coded sequence with the mature polypeptide coding sequence of FSH beta subunit gene, be built into the carrier for expression of eukaryon of restructuring goose FSH β-CTP-alpha fusion protein, with mammalian cell expression and purification of goose Fc-FSH β-CTP-alpha fusion protein is standard items; Set up the double-antibodies sandwich ELISA detecting goose FSH. |
| priorityDate | 2013-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.