http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103388006-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2013-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103388006-B |
| titleOfInvention | A kind of construction process of site-directed point mutation |
| abstract | The present invention discloses a kind of construction process of site-directed point mutation, to determine in rat target gene group sequence can the target sequence that identifies by artificial reconstructed CRISPR-Cas system; Can structure identify and guide CAS albumen to the guiding RNA sequence (guide of target gene target sequence? RNA, gRNA); The CAS egg white mixture of above-mentioned gRNA sequence and CAS protein-encoding nucleotide sequence or gRNA sequence and vivoexpression is imported rat embryo cell.The present invention without the need to building homologous recombination targeting vector, without the need to carrying out ES target practice cell screening, mutant ratio is high, easy and simple to handle, can realize multidigit point and knock out simultaneously, can significantly reduce experimental cost and shorten experimental period.<pb pnum="1" /> |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9340800-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9526784-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022197727-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11591581-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9340799-B2 |
| priorityDate | 2013-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.