http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103387967-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-36 |
| filingDate | 2012-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62c77359556e11230415f03efe1c99b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a43459d543c4d4ddcf98701aa31dc02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a6579dfbf1b520af61872c5850a5043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1864b536bdcd8abe09503de4153285e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ce88b3999eb02d88cbbbc162d070779 |
| publicationDate | 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103387967-B |
| titleOfInvention | Method for regulating and controlling bioactivity of lysozyme based on induced crystallization effect of ionic liquid |
| abstract | The invention discloses a method for regulating and controlling bioactivity of lysozyme based on the induced crystallization effect of ionic liquid. The method comprises the following steps: (1) adding water-soluble imidazolium ionic liquid into a solution A which is a sodium acetate buffer solution of NaCl with a pH value of 4 to 6.5 and mass concentration of 3 to 6%; (2) adding egg white lysozyme into liquid obtained in the step (1), wherein a supersaturation ratio of the lysozyme is 1 to 3 at a temperature of 20 to 30 DEG C; and (3) cooling the liquid obtained in the step (2) with stirring until a crystal is precipitated, carrying out crystal growing in turbidity, then carrying out cooling so as to allow a crystallization solution to be obtained, filtering the crystallization solution, drying the crystal so as to obtain a lysozyme crystal product and preserving the lysozyme crystal product at a temperature of 4 DEG C. The method provided by the invention has the advantages of easy realization of process control and convenience in operation. The prepared lysozyme crystal product has uniform granularity, a small variable coefficient, main granularity of more than 100 mu m, mass yield of more than 90%, intact appearance and substantially improved relative activity. |
| priorityDate | 2012-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.