http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103382502-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103382502-B |
| titleOfInvention | A kind of RT-PCR method integrating restriction enzyme removal DNA pollution |
| abstract | The invention provides a kind of RT-PCR method integrating restriction enzyme removal DNA pollution.With the amplification region of the sequence of wherein a kind of restriction enzyme enzyme recognition site as RT-PCR on the method select target RNA, after reverse transcription completes, endonuclease reaction and PCR reaction are incorporated in an individual system and successively carry out continuously; Because cDNA sequence and target RNA form RNA/DNA heteroduplex, can not be cut, and the pcr amplification product of the genomic dna of trace or crossed contamination is all double-stranded DNA, can be cut, and be inactivated in the process of PCR denaturation due to restriction enzyme, therefore, while the genomic dna avoiding trace or amplified production crossed contamination interference, the pcr amplification efficiency of cDNA is unaffected.Do not need to carry out DNase ferment treatment in the method RNA sample set-up procedure, and greatly reduce the generation of PCR primer crossed contamination yet. |
| priorityDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 226.