http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103361440-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50841fbcdd36783f8592938404f22ea8 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd538da3cf6ee5a6413fb6ca5439c356 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62915f6b6daf68695766d6dededaee32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4bafc6c64184f6e4733b95474d702c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d7964b75d372421b105a8552c68d140 |
publicationDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103361440-B |
titleOfInvention | LCT gene polymorphism detection method for piglet lactase digestive symptoms and applications of the method |
abstract | The invention discloses a LCT gene polymorphism detection method for piglet lactase digestive symptoms. Main steps of the method comprise a step of extracting tissue DNA from pig ears, designing primers, obtaining a sequence of a LCT gene 5'UTR section, and performing a PCR product clone sequencing process; a step of directly sequencing PCR-SSCP and a PCR product to perform polymorphism detection; and a step of analyzing correlation between polymorphism and piglet lactase non-persistent diarrhea symptoms. Results show that the LCT gene 5'UTR polymorphism and the piglet lactase non-persistent diarrhea are significantly correlated. The invention also discloses two pairs of primer sequences used for amplifying the LCT gene 5'UTR polymorphism, three base mutation sites of the LCT gene 5'UTR gene segment, and detection, evaluation and applications of the polymorphism for the piglet lactase non-persistent diarrhea symptoms. The detection method provides a novel molecular mark for the pig marker-assisted selection and pig breeding. |
priorityDate | 2013-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 150.