http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103361306-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate | 2013-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103361306-B |
| titleOfInvention | Pluripotent stem cell is separately cultured in mammalian tissues |
| abstract | The present invention provides pluripotent stem cells in mammalian tissues to be separately cultured, and belongs to technical field of stem cell culture.Under sterile, the separation of tissue mechanical separation method, culture sheep fetal kidney tissue primary cell are cultivated 48 hours with culture solution II 37；It washs after cell clone appearance, is cultivated 18 hours after culture solution III is added；The cell being collected by centrifugation culture solution II suspension cell, is inoculated in culture dish, cell clone occurs；It is frozen after 3~4 generations of biography spare.Culture solution I component includes DMEM, L-Glutamine, nonessential amino acid, Sodium Pyruvate, micro beta -mercaptoethanol, LIF, bFGF；Culture solution II component includes culture solution I, FBS；Culture solution III component includes culture solution I and trypsase.The present invention can from mammalian tissues isolated pluripotent stem cell. |
| priorityDate | 2013-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 155.