abstract |
The invention relates to a Saccharopolyspora spinosa HBERC-25376 and its cultivation, separation method and application of active substances. The code name of Saccharopolyspora spinosa is HBERC-25376, and it is preserved in the China Center for Type Culture Collection with the preservation number CCTCC No: M2013076. The culture method is the preparation, expansion or fermentation of strains, secondary seed cultivation or fermentation, adjusting the pH of the fermentation broth to 4.5, plate and frame filtration or centrifugation to obtain two parts: supernatant and mycelia; use a thin film concentrator to concentrate the supernatant liquid, or extract the supernatant by extraction or resin adsorption. During the fermentation process, fascicillin A, nodomycin, and pepper phytophthora are produced. They have good insecticidal and bactericidal (fungal, bacterial) activities, and can be used for crop pests such as diamondback moth and cotton bollworm and crops such as Phytophthora capsici Control of fungal diseases. Its live bacteria preparation also has the potential for biological control. The toxicity of these antibiotics is very low, and some of them can be used in the prevention and treatment of animal and human diseases. Live bacterial preparations can also be made by solid fermentation or solid-liquid two-phase fermentation. |