http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103360489-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-765 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 |
| filingDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2506ed08e5e22a863f2ae9d71e04cdf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f7029618a0db40638b141e33070abdc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1486d4f037098001ea60926f86906c2 |
| publicationDate | 2015-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103360489-B |
| titleOfInvention | Method for synthesizing acid-orange II artificial antigen and method for preparing acid-orange II IgY antibody |
| abstract | The invention discloses a method for synthesizing an acid-orange II artificial antigen. The method is characterized by comprising the following steps of: weighing acid-orange II, adding acetone to dissolve the acid-orange II, adding succinic anhydride and anhydrous pyridine, and heating for refluxing for 24 hours; (2) removing the acetone by rotary evaporation, drying to obtain a layer of substance in orange color at the bottom of a distillation flask, adding DMF (dimethyl formamide) to dissolve, adding NHS (N-hydroxysuccinimide) and DCC (dicyclohexylcarbodiimide), stirring by a magnetic stirrer at room temperature to obtain a reaction liquid, centrifuging, and removing a precipitate to prepare an acid-orange II activating intermediate product; (3) weighing BSA (Bovine serum albumin) or OVA (ovalbumin), dissolving the BSA or the OVA in PBS (poly butylene succinate), dropwise adding the acid-orange II activating intermediate product into a BSA-PBS solution or an OVA-PBS solution while stirring by the magnetic stirrer, and stirring overnight; (4) adding a reaction liquid obtained by stirring overnight into a dialysis bag, and dialyzing for 72 hours in the PBS in a refrigerator, wherein the dialysate is changed every 8 hours; and (5) centrifuging the reaction liquid in the dialysis bag after finishing dialyzing, and removing a precipitate to obtain the acid-orange II artificial antigen. |
| priorityDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.