http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103353505-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527c9954477fb1ad4df592bfd9cdaa00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-90 |
| filingDate | 2013-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60a56d4c8fe1091c2bd0bbd0ee6f6bbf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a1a15689654131a61ca798a27bd7258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_273c337c2216f9cfbea622c62c095dac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_efa1e518e2d386e25e9a17f5e8758367 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df29cf93439c984d15ae56902e8d3543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee391a5b64024ca58363f508f3a27632 |
| publicationDate | 2015-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103353505-B |
| titleOfInvention | Method for distinguishing DendrobiumofficinaleKimuraetMigo polysaccharides through adopting thin layer chromatography |
| abstract | The invention provides a method for distinguishing DendrobiumofficinaleKimuraetMigo polysaccharides through adopting the thin layer chromatography, which includes the following steps: preparing a test solution and a reference substance and adopting the thin layer chromatography for distinguishing, wherein the thin layer chromatography includes the following steps: absorbing 10 muL of the reference substance and 10 muL of the test solution respectively and dripping the reference substance and the test solution on a microcrystalline cellulose thin layer plate respectively, taking a mixed solution of isoamylol, dichloromethane, ammonia water, succinic acid, absolute ethyl alcohol and water as a developing solvent for developing, taking out and drying the microcrystalline cellulose thin layer plate, spraying the plate through color developing agent, heating for 5 min at the temperature of 105 DEG C, and viewing under white light, wherein isoamylol : dichloromethane : ammonia water : succinic acid : absolute ethyl alcohol : water equals to 11 : 25 : 18 : 15 : 15 : 10, and in the chromatography of a hydrolyzing sample of DendrobiumofficinaleKimuraetMigo, spots with the same colors are displayed at positions corresponding to the chromatography of the reference substances D-glucose and D-mannose. The method has the advantages that the operation is simple and convenient, the experimental cost is low, the separating effect is quick and accurate, and a reference for further quality control of DendrobiumofficinaleKimuraetMigo is provided. |
| priorityDate | 2013-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.