http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103352256-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d13b253bd8988165c65d816362f7316 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
filingDate | 2013-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3929156a76d2a4b44a53ed21751006c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4176cede47052fbc0171666415eaadc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52b50e8aba433f646a259a5882d454f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31bb0d021aa3fa06fe1bcafffae96f93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f809f851dd46aace1b02dfd864f651b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ec951934f5cf7f512cadeb8eddae26d |
publicationDate | 2013-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103352256-A |
titleOfInvention | Excellent winter sport athlete placenta cDNA library and construction method thereof |
abstract | The present invention relates to an excellent winter sport athlete placenta tissue cDNA library and a construction method thereof, wherein excellent winter sport athlete placenta tissue is adopted as a template, and total RNA extraction, double-stranded cDNA synthesis, digestion through proteinase K, Sfi1 enzyme digestion, linking to a vector, and lambda phage packaging are performed to finally obtain the excellent winter sport athlete placenta tissue cDNA library with characteristics of high titer, high recombination rate, large insertion fragment range and high quality. Excellent winter sport athletes have characteristics of high skills, high physical agility, and high endurance, and sport genes of the excellent winter sport athletes are extremely valuable genetic resources, such that it is necessary to establish the excellent winter sport athlete placenta tissue cDNA library so as to conveniently screen useful genes. The invention belongs to the cross discipline field of sports science, human resource methodology, cell biology and molecular biology. |
priorityDate | 2013-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.