http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103333967-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 |
| filingDate | 2013-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103333967-B |
| titleOfInvention | A kind of nucleic acid detection method based on microfluidic microbead array chip |
| abstract | The invention discloses a kind of nucleic acid detection method based on microfluidic microbead array chip。The present invention is mainly: in microsphere surface, specificity capture probe and electron rich proteopexy are formed functionalized microsphere, and is fixed in micro-fluidic chip detection zone by this microsphere；Using horseradish peroxidase and detection probe modification on nano-particle as signal labelling；The detection zone that target nucleic acid sequence and nano-particle flow into microfluidic microbead array detection chip is reacted with micropearl array；By multienzyme nanowire signal amplifying technique, biotinylated tyramine is attached on the electron rich albumen of microsphere surface, the quantum dot of affinity element labelling is flowed into after eluting, this quantum dot can with microsphere surface electron rich albumen on combine biotin identification and fix, carry out detection by quantitative by fluorescence。The sensitivity that the present invention can solve to exist in current method for nucleic acid analysis is low, testing process is complicated and is difficult to the problem that micro-example is analyzed, and promotes the further development of this technical field。 |
| priorityDate | 2013-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.