http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103333938-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20 |
| filingDate | 2013-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103333938-B |
| titleOfInvention | The hepatitis B surface antigen of recombinant Saccharomyces cerevisiae expression and production method, Hepatitis B virus vaccine and production method thereof |
| abstract | The present invention discloses the production method of the hepatitis B surface antigen that a kind of recombinant Saccharomyces cerevisiae is expressed, including: triton is removed in the antigen XAD 4 post circulation extracted, obtains triton antigen sample liquid；By aperture be 1000A, granular size be that the macro porous silica gel of 35 μm～70 μm loads in chromatographic column, and by the phosphate buffer balance that pH value is 7.6 ± 0.2；To go triton antigen sample liquid NaOH adjust pH value to after 7.6 ± 0.2, loading macro porous silica gel post；Wash miscellaneous with the phosphate buffer that pH value is 7.2 ± 0.2, at a temperature of 47～49 DEG C with the borate buffer that pH value is 8.7 ± 0.2 to wash miscellaneous after macro porous silica gel post carry out eluting process and collect eluent, to eluent be concentrated by ultrafiltration, obtain clarify antigen.The invention also discloses hepatitis B surface antigen, Hepatitis B virus vaccine and production method thereof that corresponding recombinant Saccharomyces cerevisiae is expressed.The present invention can be substantially reduced contamination of products probability, alleviate hand labor intensity, saving equipment investment and maintenance cost, minimizing equipment occupation space, shortens the production time. |
| priorityDate | 2013-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.