http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103333883-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2013-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103333883-B |
| titleOfInvention | A kind of high efficiency extraction is used for the method for the groundwater microbial DNA of pcr amplification |
| abstract | The invention discloses the method for a kind of high efficiency extraction for the groundwater microbial DNA of pcr amplification, steps of the method are: the first step collects groundwater microbial, collect microorganism with aseptic millipore filtration, then with the damping fluid containing cetyl trimethylammonium bromide, microorganism is eluted obtained microorganism cells liquid; Second step cracking groundwater microbial cell, adds N,O-Diacetylmuramidase in microorganism cells liquid and Proteinase K carries out lysis, centrifugal after warm water water-bath, obtains lysis supernatant liquor; 3rd step purifying cells lysate, after lysis supernatant liquor is carried out repeatedly centrifugal purification, is slightly carried DNA solution; 4th step purifying microbe genome DNA, by slightly carry DNA solution carry out centrifugal purification, by volumetric concentration be the ethanolic extraction of 70% after obtain the microbe genome DNA of purifying.The inventive method high efficiency extraction can go out high-quality groundwater microbial genomic dna, and simple to operate, DNA purity is high. |
| priorityDate | 2013-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.