http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103333817-B

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filingDate 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b304cf80409717a92a56644a913de1dc
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publicationDate 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103333817-B
titleOfInvention Method for magnetically separating yersinia enterocolitica (YE)
abstract The invention relates to the technical field of biology and discloses a method for enriching and separating yersinia enterocolitica (YE). The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacteria in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.
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