http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103323556-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7ee07409c10fa369c4a4754382c07be |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2013-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af0ce5bab3badbaa1ee89e62c3f03fcf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6b029ebe5b7c9709ed05fe53b40dcf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bf107d8c78d3ab80a9906dcbea00f52 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103323556-B |
| titleOfInvention | Method for fast detecting concentration of Escherichia coli in water body by adopting high-performance liquid chromatography method |
| abstract | The invention discloses a method for fast detecting concentration of Escherichia coli in a water body by adopting a high-performance liquid chromatography method. The method comprises the following steps of: 1) obtaining a plurality of Escherichia coli solutions with different known concentrations; 2) determining the compliance relation of response values between Escherichia coli and 2,5-dihydroxy-benzoic acid on a high-performance liquid chromatography instrument as a linear relation according to the following chromatography conditions, i.e., a chromatographic column is a C18 chromatographic column, a flowing phase comprises liquid A and liquid B according to the volume ratio of (10-20):(90-80), wherein liquid A is acetonitrile, the liquid B is a phosphoric acid water solution with volume percentage of 0.03-0.075; conducting isocratic elution; detecting the wavelength of 205-235nm; and 3) introducing a water body sample to be detected, and reading the concentration of Escherichia coli in the water body to be detected according to responded concentration value of the 2,5-dihydroxy-benzoic acid through the known linear relation curve. The method is simple to operate, and the detection only lasts for three hours; and the detection limit can achieve 10,000cfu/mL. |
| priorityDate | 2013-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.