http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103320434-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e15f2f4d358ad3a715b22bc2b2badf5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cc4e13141d402ceb885f368dfa042348 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a65dae30f90bafc40aa9b11e195fa5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2a1fd137b173fe1cf49fd7e1006045d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdd70322efc1ad36fec85ae2f788a015 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c52dd9639b9dfac6c8c1dbc3fd8c63f5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_597e699c26064a057901f6283a706917 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_775925cc246ce744e7c8b257e6ac827b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71f856f296c42eb257fb5edc0962336b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d263c51f4bacf57bcf454714133b54b0 |
| publicationDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103320434-B |
| titleOfInvention | Salmonella LAMP (loop-mediated isothermal amplification) primer group and kit and detection method |
| abstract | The invention discloses a Salmonella LAMP (loop-mediated isothermal amplification) primer group and kit and a detection method, belonging to the field of a molecular biological detection method for food safety. The Salmonella LAMP primer group designed by the invention comprises a detection primer group and an internal standard primer group; and the detection kit comprises the Salmonella LAMP primer group, a DNA (deoxyribonucleic acid) polymerase, an LAMP reaction solution, a fluorescent nucleic acid dye, an internal standard, a positive control and a negative control. The detection method comprises the following steps: extracting a sample to be detected, and amplifying the sample template at 63-65 DEG C for 30-45 minutes. Thus, whether the sample to be detected contains Salmonella can be detected, and meanwhile, whether a false negative detection result exists can be judged according to whether the detection internal standard primer group is amplified. The invention has the advantages of high speed, high efficiency, simple operation process, high specificity, high sensitivity and the like, is suitable for on-site detection, can effectively prevent the occurrence of false negative, and is suitable for popularization and application. |
| priorityDate | 2013-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.