http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103308494-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2012-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103308494-B |
| titleOfInvention | The method of spike is carried out to the absorbed albumen of energy in Chinese medicine |
| abstract | The invention discloses and a kind ofly can carry out the method for spike by absorbed albumen in Chinese medicine.Method of the present invention comprises the steps: that Chinese medicine protein extract fluorophor marks by (1); (2) experiment model animal is given by the Chinese medicine protein extract gavage after mark; (3) gather the serum of described experiment model animal, carry out non-denatured protein electrophoresis after concentrated, gel is cut into some blob of viscoses by vertical electric field direction, detects fluorescence intensity and the coefficient of diffusion of each blob of viscose; (4) carry out film dosim with trypsase respectively by screening the blob of viscose obtained, then enzymolysis product is carried out the sieve chromatography under real-time fluorescence monitoring, recovery has the peak of fluorescence signal and carries out mass spectroscopy; (5) determining according to the result of mass spectroscopy can absorbed albumen.Method of the present invention will make tremendous contribution for disclosing the large molecular mechanisms of action of Chinese medicine, and for promoting traditional Chinese medicine and pharmacy development based theoretical. |
| priorityDate | 2012-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.