http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103305546-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 |
| filingDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6757a0c46f00cd982aa19813ecef0301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26e4b975f55b648b192334f1705b8c16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83b8b0afa8786d6580202eb15e52e567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5048ecb8ad88e868a43c2ae1d575d63d |
| publicationDate | 2014-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103305546-B |
| titleOfInvention | pGreen Max1 eukaryotic expression vector and preparation method thereof |
| abstract | The invention relates to a pGreen Max1 eukaryotic expression vector and a preparation method thereof in the technical field of molecular biology. Mex and IRES-EGFP segments are recombined with the eukaryotic expression vector to obtain a sequence 1; and the total length of the sequence 1 is 8003 bp, wherein a Mex segment of 647 bp is inserted between the 176th bp and the 2415th bp and an IRES-EGFP sequence of 1309 bp is inserted between the 3120th bp and the 4428th bp; and the preparation method comprises the following steps of: obtaining Mex by cloning from a plasmid by a PCR (Polymerase chain reaction) method by virtue of a designed primer, and directionally inserting the PCR product into the expression vector through a Sma I site, and inserting IRES-EGFP into the vector through BamHi and NotI double digestion, thereby establishing the recombinant pGreen Max1 eukaryotic expression vector. The vector provided by the invention is applied to obtaining a target protein or antibody from an eukaryotic cell, and provides protein expression for screening of high-yield mammalian cell lines and a mark for screening. |
| priorityDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.