http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103305460-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4e867d6726e467b5f8706629d7875307 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate | 2013-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a5f7f443efc69471ef85b3cf7fc4bd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e7a657a232eefca91f170f41fe13b20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68953983d5d01ddf044fde4ed2bc2420 |
| publicationDate | 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103305460-B |
| titleOfInvention | Method for separating and culturing human epidermal stem cells |
| abstract | The invention provides a method for separating and culturing human epidermal stem cells, relating to the separation and culture of cells. The method comprises the step of cold-digesting for 4-8 hours under the condition of 4 DEG C through 0.25% Try for subculturing. Compared with the prior art, the method has the beneficial effects that the activity of cells obtained by using the separating and culturing method is high and can be improved to more than 90% compared with the traditional digestion method; furthermore, the number of living cells is more and can be improved to 1.8*10<5>/ml from 0.9*10<5>/ml of the traditional digestion method, the adherence effect is good in subculturing, and the cell adherence rate can be increased to 88% from 62%; simultaneously, epidermal cells digested by utilizing the traditional digestion method can only be generally subcultured by two to three generations; and after utilizing the method, the cells can be subcultured by six to eight generations, and the subculturing times can also be improved. |
| priorityDate | 2013-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.