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grantDate 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103305440-B
titleOfInvention Fast magnetic separation method of mycobacterium tuberculosis (MT)
abstract The invention discloses a method for enrichment separation of mycobacterium tuberculosis (MT), and better provides a basis to the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the following steps of: performing covalent coupling of a hyperbranched polymer and an antibody; coating a long-chain biotin molecule with the antibody-modified hyperbranched polymer; capturing target bacteria in sample liquid by use of the hyperbranched polymer co-modified by the antibody and the long-chain biotin; identifying streptavidin-modified nano magnetic beads, and coupling with the long-chain biotinylation hyperbranched polymer in the sample liquid; separating and re-suspending the captured bacteria, and the like, wherein the re-suspension liquid can be directly subjected to follow-up analysis. Compared with a traditional magnetic bacterium separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicate matrix, and improves the separation efficiency of target bacteria in a sample.
priorityDate 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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