http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103304679-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a6268ac9a5f07efa6faf2acc67fdb6a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 |
| filingDate | 2013-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57a65bcad39d7abbe69f69f324764eea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3670ebbb43dfe0ca9d59a8f9ba8a99b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c252bf58758b6a5455800c51e63fe540 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e59b4684566cbdb3b1570cc9bfb03ec3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e9e85bd479f5358138b5f0a7937e869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8749caa56480183b6976c0c480b01ade http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cae814e0d677a8ae18dfea415ff60e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4730ce1f231c8544a79c98162d4301c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c815ff5b30e1cc116e18a31ceca9cef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22333d3195ec94e04da3bd9cb0ba8dc0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1cf9cc1c3437088c9d04673c332a7c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39c861201773a5a965f417a145ea165a |
| publicationDate | 2015-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103304679-B |
| titleOfInvention | Large-scale preparing and purifying method of bacteria O-specific polysaccharide |
| abstract | The invention discloses a large-scale preparing and purifying method of bacteria O-specific polysaccharide. The large-scale preparing and purifying method comprises the following steps of: (1) hydrolyzing of lipopolysaccharide into bacteria O-specific polysaccharide raw sugar; (2) the bacteria O-specific polysaccharide raw sugar purifying: first-time ultra-filtration, alcohol fractional precipitation, the second-time ultra-filtration and anion exchange; the anion exchange: adding a buffer solution to an ultra-filtration concentrated solution which is obtained by the second-time ultra-filtration, sampling to an anion exchange column, and using the buffer solution to leach the anion exchange column until ultraviolet absorption value is not lowered; using eluting liquid to elute, and collecting the eluting peak, i.e., a target solution; and (3) concentrating and freezing the target solution to obtain the purified bacteria O-specific polysaccharide. The large-scale preparing and purifying method of the bacteria O-specific polysaccharide disclosed by the invention is convenient to operate, low in preparation cost, larger in treatment capacity, suitable for large-scale and industrial production. Moreover, the product obtained by production is low in impurity content, and high in purity, so that the requirements of furthermore developing human-use vaccines are achieved. |
| priorityDate | 2013-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.