http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103299907-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b7dec7a3442e878cdbf561c8433b8a6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2013-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2612d34b9d210be93ca6d0dd4cdab01 |
| publicationDate | 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103299907-B |
| titleOfInvention | Method for conserving in-vitro body of radix tetrastigme germplasm resources and recovering growth after conservation |
| abstract | The invention discloses a method for conserving in-vitro body of radix tetrastigme germplasm resources and recovering growth after conservation. The stem tip of the radix tetrastigme with axillary bud is taken as the in-vitro body, the method comprises the following steps of: carrying out surface sterilization by 8% sodium hypochlorite at 8min, washing by aquae sterilisata at twice, sterilizing by 0.1% L of mercury, washing by aquae sterilisata at eight times, and transferring the stems which are cut into about 1cm into a culture medium 1; transferring the germinal axillary bud into a culture medium 2 for expanding propagation after 20d; transferring the proliferated adventitious bud into a culture medium 3 for root induction after 30d; and transferring the formed test-tube plantlet into a culture medium 4 after 20d, and conserving under the conditions of 8+/-1 DEG C and 600Lux illumination. The method for conserving the in-vitro body of the radix tetrastigme germplasm resources and recovering the growth after conservation disclosed by the invention is long in germplasm conservation time, stable in germplasm quality, simple in germplasm conservation technology, stable and reliable in technology, high in repeatability, and suitable for the large-scale conversation of the radix tetrastigme germplasm resources. |
| priorityDate | 2013-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.