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filingDate 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31f217a49f08a84d355ad7da720353fa
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publicationDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103290126-B
titleOfInvention Molecular marker for distinguishing cabbage eIF (iso) 4G gene wild type and mutant and application thereof
abstract The invention discloses a site-specific dominant ASM marker for distinguishing cabbage eIF (iso) 4G wild type and a mutant, the marker which is related to wild type site detection is named as ASM-I4E. G, the fragment size is 1526bp, and the marker is as shown in SEQ (sequence) ID (identity) No. 1. Primers for identifying the specific dominant molecular marker are as follows: the forward primer PF1 is as follows: 5'-TTTTTTGGTTGTTGGAGATTTTG-3'; the reverse primer PR1 is as follows: 5'-GGTACTTCAGCTTTGACGAGGAC-3'; and the primers are as shown in SEQ ID No. 2 and SEQ ID No. 3. The marker can be applied to cabbage species resource identification and breeding assisted selection: a pair of primers of the ASM marker can be utilized for performing PCR (polymerase chain reaction) amplification on DNA (deoxyribonucleic acid) of a genome of an individual to be tested so as to detect whether an amplification fragment exists or not, if a ribbon can be amplified, the individual is of a homozygous wild type or a heterozygote, and if the ribbon can not be amplified, the individual is of a mutant type. According to the application disclosed by the invention, the screening means can be greatly simplified, the transferring years can be shortened, and the blindness in selection of a conventional breeding method can be avoided.
priorityDate 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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