http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103290117-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abd156263f0d78fc96518fafc94f377f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f340693b77c232f32e35471f7144e653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b64eef46c149779bd233ab5bc587c31e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ace71305328a29380354a2b268558853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6d32a9b91f7f428f0142dc8f45cd21a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b3d85364cf22319fd1919c56c1236f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b820267f09f92f54e87006ed4fdc1bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8694488abdfadec40fcabda894ef5b5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a75af6291403cbb53bb677cc3c1bcebb |
| publicationDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103290117-B |
| titleOfInvention | Method for identifying protoplast monokaryon mating types of lepista sordida and special primer pair SR-4*2 thereof |
| abstract | The invention discloses a method for identifying the protoplast monokaryon mating types of lepista sordida and a special primer pair SR-4*2 thereof. The method comprises the following steps of: with genome DNAs (deoxyribonucleic acids) of the protoplast monokaryons of two plants of lepista sordida to be identified as templates, performing PCR (Polymerase Chain Reaction) amplification by using a PCR primer pair composed of two single chain DNAs as shown in SEQ ID No.1 and SEQ ID No.2, and detecting the sizes of the obtained PCR products; if the PCR products of the protoplast monokaryons of the two plants of lepista sordida to be identified both contain or do not obtain 500 bp-800 bp of DNA segments, indicating that the mating types of the protoplast monokaryons of the two plants of lepista sordida are the same; and if one plant contains 500 bp-800 bp of DNA segments, while the other plant contains no 500 bp-800 bp of DNA segments, indicating that the mating types of the protoplast monokaryons of the two plants of lepista sordida are different. |
| priorityDate | 2013-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.